ER anticuerpos | 48 (2 knockout-validated) products in Validated Antibody Database; 72 cited in the literature; 480 total from 32 suppliers

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Estrogen Receptor alpha Monoclonal Antibody (SP1)
Invitrogen
catálogo: MA5-14501

total number of products: 45

conejo monoclonal (Sp1)
reactividad: Human papillomavirus, humanos, ratón, rata, ovejas
aplicación: western blot, inmunohistoquímica, inmunocitoquímica, inmunoprecipitación, citometría de flujo, immunohistochemistry - paraffin section, reverse phase protein lysate microarray, otro
citations: 315

Formalin-fixed, paraffin-embedded human breast carcinoma stained with Estrogen Receptor using peroxidase-conjugate and AEC. Note nuclear staining of tumor cells
cantidad: 500µL
Precio: US 710
al proveedor
Estrogen Receptor alpha Monoclonal Antibody (SP1)
Invitrogen
catálogo: MA1-39540

total number of products: 45

conejo monoclonal (Sp1)
reactividad: Human papillomavirus, humanos, ratón, rata, ovejas
aplicación: western blot, inmunohistoquímica, inmunocitoquímica, inmunoprecipitación, citometría de flujo, immunohistochemistry - paraffin section, reverse phase protein lysate microarray, otro
citations: 312

cantidad: 1 mL
Precio: US 1116
al proveedor
Estrogen Receptor alpha Monoclonal Antibody (SP1)
Invitrogen
catálogo: MA1-39539

total number of products: 45

conejo monoclonal (Sp1)
reactividad: Human papillomavirus, humanos, ratón, rata, ovejas
aplicación: western blot, inmunohistoquímica, inmunocitoquímica, inmunoprecipitación, citometría de flujo, immunohistochemistry - paraffin section, reverse phase protein lysate microarray, otro
citations: 312

cantidad: 7 mL
Precio: US 380
al proveedor
ERalpha(F-10)anticuerpos
Santa Cruz Biotechnology
catálogo: sc-8002

total number of products: 20

ratón monoclonal (F-10)
reactividad: humanos, ratón
aplicación: western blot, ELISA, inmunohistoquímica, inmunocitoquímica, inmunoprecipitación, immunohistochemistry - paraffin section, western blot knockout validation
citations: 112

cantidad: 200 µg/ml
Precio: 279 USD
al proveedor
ERalpha(D-12)anticuerpos
Santa Cruz Biotechnology
catálogo: sc-8005

total number of products: 20

ratón monoclonal (D-12)
reactividad: humanos, ratón
aplicación: western blot, ELISA, inmunohistoquímica, inmunocitoquímica, inmunoprecipitación, immunohistochemistry - paraffin section
citations: 51

cantidad: 200 µg/ml
Precio: 279 USD
al proveedor
ERalpha(C-311)anticuerpos
Santa Cruz Biotechnology
catálogo: sc-787

total number of products: 20

ratón monoclonal (C-311)
reactividad: humanos, ratón, rata, Sus
aplicación: western blot, inmunohistoquímica, inmunocitoquímica, inmunoprecipitación, immunohistochemistry - paraffin section
citations: 26

cantidad: 200 µg/ml
Precio: 279 USD
al proveedor
Anti-Estrogen Receptor alpha antibody [SP1]
Abcam
catálogo: ab16660

total number of products: 21

conejo monoclonal (Sp1)
reactividad: humanos
aplicación: western blot, inmunohistoquímica, inmunocitoquímica, immunohistochemistry - paraffin section
citations: 19

cantidad: 1 ml, 100 µl, 500 µl
Precio:
al proveedor
Anti-Estrogen Receptor alpha antibody [SP1], prediluted
Abcam
catálogo: ab27595

total number of products: 21

conejo monoclonal (Sp1)
reactividad: humanos, ratón
aplicación: western blot, inmunohistoquímica, inmunocitoquímica, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 12

cantidad: 7 ml
Precio:
al proveedor
Anti-Estrogen Receptor alpha antibody [E115] - ChIP Grade
Abcam
catálogo: ab32063

total number of products: 21

conejo monoclonal (E115)
reactividad: humanos, ratón, rata
aplicación: western blot, inmunocitoquímica, citometría de flujo, inmunoprecipitación de la cromatina , immunohistochemistry - paraffin section
citations: 12

cantidad: 10 µl, 100 µl
Precio:
al proveedor
ER
Proteintech Group
catálogo: 21244-1-AP

conejo policlonal
reactividad: humanos, ratón, rata, vacas
aplicación: western blot, ELISA, inmunohistoquímica, inmunocitoquímica, immunohistochemistry - paraffin section
citations: 20

The ER antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human ER. This antibody recognizes human,mouse,rat antigen. The ER antibody has been validated for the following applications: ELISA, WB, IHC analysis.
cantidad: 150 ul
Precio: 299 USD
al proveedor
Estrogen receptor α monoclonal antibody (C-542)
Enzo Life Sciences
catálogo: ADI-SRA-1010-D

total number of products: 5

ratón monoclonal (C-542)
reactividad: humanos, ratón, rata
aplicación: western blot, inmunohistoquímica, inmunocitoquímica, inmunoprecipitación
citations: 5

Human Estrogen Alpha Receptor domains.

Western blot analysis of Estrogen Receptor alpha mAb: Lane 1: MWM, Lane 2: Estrogen Receptor 1 (GST-tagged ER1 protein control runs at 92 kDa).
cantidad: 50 µg
Precio: 180 USD
al proveedor
Estrogen receptor monoclonal antibody (h-151)
Enzo Life Sciences
catálogo: ADI-SRA-1000-D

total number of products: 5

ratón monoclonal (h-151)
reactividad: humanos, rata
aplicación: western blot, inmunocitoquímica, inmunoprecipitación
citations: 4

Western blot analysis of ESR1: Lane 1: MWM, Lane 2: Estrogen Receptor 1 (GST-tagged ER1 protein control runs at 92 kDa)

Human Estrogen Receptor domains.
cantidad: 50 µg
Precio: 169 USD
al proveedor
Estrogen receptor monoclonal antibody (h-151)
Enzo Life Sciences
catálogo: ADI-SRA-1000-F

total number of products: 5

ratón monoclonal (h-151)
reactividad: humanos
aplicación: western blot, inmunocitoquímica, inmunoprecipitación
citations: 4

Western blot analysis of ESR1: Lane 1: MWM, Lane 2: Estrogen Receptor 1 (GST-tagged ER1 protein control runs at 92 kDa)

Human Estrogen Receptor domains.
cantidad: 200 µg
Precio: 381 USD
al proveedor
ER alpha/NR3A1 Antibody
R&D Systems
catálogo: PP-H4624-00

total number of products: 6

ratón monoclonal (H4624)
reactividad: humanos
aplicación: western blot, ELISA, inmunohistoquímica, inmunoprecipitación
citations: 2

cantidad: 100 ug
Precio: 399 USD
al proveedor
ER alpha/NR3A1 Antibody
R&D Systems
catálogo: AF5715

total number of products: 6

ovejas policlonal
reactividad: humanos
aplicación: western blot, inmunohistoquímica, proximity ligation assay
citations: 1

ER alpha/NR3A1 in Human Endometrial Cancer Tissue. ER alpha/NR3A1 was detected in immersion fixed paraffin-embedded sections of human endometrial cancer tissue using Sheep Anti-Human ER alpha/NR3A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5715) at 10 ╡g/mL overnight at 4 ░C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013 ). Tissue was stained using the Anti-Sheep HRP-DAB Cell Tissue Staining Kit (brown; Catalog # CTS019 ) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections .
cantidad: 100 ug (also 25 ug)
Precio: 369 USD
al proveedor
ER alpha/NR3A1 Antibody
R&D Systems
catálogo: MAB57151

total number of products: 6

ratón monoclonal (681118)
reactividad: humanos, ratón, rata
aplicación: western blot
citations: 1

Detection of Human, Mouse, and Rat ER alpha/NR3A1 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line. PVDF Membrane was probed with 0.05 ╡g/mL of Human ER alpha/NR3A1 Monoclonal Antibody (Catalog # MAB57151) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007 ). A specific band was detected for ER alpha/NR3A1 at approximately 65 to 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 .
cantidad: 100 ug (also 25 ug)
Precio: 289 USD
al proveedor
Mouse anti Human ESTROGEN RECEPTOR ALPHA
Bio-Rad
catálogo: MCA1799

total number of products: 6

ratón monoclonal (6F11)
reactividad: humanos
aplicación: western blot
citations: 2

Western blot analysis of MCF-7 human breast adenocarcinoma whole cell lysate probed with Mouse anti Human estrogen receptor alpha antibody (MCA1799) followed by HRP conjugated Goat anti Mouse IgG, visualized by chemiluminescence
cantidad: 1 ml
Precio: 1301 USD
al proveedor
Mouse anti Human ESTROGEN RECEPTOR ALPHA
Bio-Rad
catálogo: MCA1799T

total number of products: 6

ratón monoclonal (6F11)
reactividad: humanos
citations: 1

Western blot analysis of MCF-7 human breast adenocarcinoma whole cell lysate probed with Mouse anti Human estrogen receptor alpha antibody (MCA1799) followed by HRP conjugated Goat anti Mouse IgG, visualized by chemiluminescence
cantidad: 0.1 ml
Precio: 145 USD
al proveedor
Mouse anti ESTROGEN RECEPTOR ALPHA
Bio-Rad
catálogo: VMA00231

total number of products: 6

ratón monoclonal
reactividad: humanos, ratón, rata
aplicación: western blot

Western blot analysis of whole cell lysates probed with estrogen receptor alpha antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P ) and visualized on the ChemiDoc MP with 3 second exposure. Arrow points to estrogen receptor alpha (molecular weight 66 kDa)
cantidad: 100 µl
Precio: 314 USD
al proveedor
Estrogen Receptor-M-NM-1 antibody (mAb)
Active Motif
catálogo: 61035

ratón monoclonal (F3)
reactividad: humanos
aplicación: western blot, inmunocitoquímica, inmunoprecipitación, de EMSA , inmunoprecipitación de la cromatina
citations: 2

cantidad: 100 ug
Precio: 350 USD
al proveedor
ESR1 (Estrogen Receptor 1/ER) mouse monoclonal antibody, clone OTI1B1 (formerly ...
OriGene
catálogo: TA503759

total number of products: 31

ratón monoclonal (OTI1B1)
reactividad: humanos
aplicación: western blot, inmunohistoquímica
citations: 2

cantidad: 100 ul
Precio: 379 USD
al proveedor
ESR1 (Estrogen Receptor 1/ER) mouse monoclonal antibody, clone OTI2E10 (formerly ...
OriGene
catálogo: TA506414

total number of products: 31

ratón monoclonal (OTI2E10)
reactividad: humanos
aplicación: western blot, inmunohistoquímica

cantidad: 100 ul
Precio: 360 USD
al proveedor
Rabbit polyclonal Estrogen Receptor-a (Ab-537) antibody
OriGene
catálogo: TA311731

total number of products: 31

conejo policlonal
reactividad: humanos
aplicación: western blot, inmunohistoquímica

cantidad: 100 ul
Precio: 335 USD
al proveedor
Purified anti-Estrogen Receptor
BioLegend
catálogo: 916201

rata monoclonal (D75)
reactividad: humanos
aplicación: immunohistochemistry - paraffin section
citations: 1

cantidad: 1 ml
Precio: 422 USD
al proveedor
ESR1 policlonal anticuerpos
Abnova
catálogo: PAB12620

total number of products: 42

conejo policlonal
reactividad: humanos
aplicación: western blot, ELISA, inmunohistoquímica, inmunoprecipitación
citations: 1

cantidad: 100 ug
Precio:
al proveedor
ESR1 purified MaxPab rabbit polyclonal antibody (D01P)
Abnova
catálogo: H00002099-D01P

total number of products: 42

conejo policlonal
reactividad: humanos
aplicación: western blot, inmunocitoquímica

cantidad: 100 ug
Precio:
al proveedor
ESR1 monoclonal antibody (M01), clone 2F8
Abnova
catálogo: H00002099-M01

total number of products: 42

ratón monoclonal (2F8)
reactividad: humanos
aplicación: western blot, ELISA, inmunocitoquímica

cantidad: 100 ug
Precio:
al proveedor
ER Alpha / Estrogen Receptor Antibody (C-Terminus) IHC-plus™ LS-B2664
LifeSpan Biosciences
catálogo: LS-B2664

total number of products: 181

conejo policlonal
reactividad: humanos
aplicación: inmunohistoquímica, inmunocitoquímica, immunohistochemistry - paraffin section
citations: 1

Anti-Estrogen Receptor antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
cantidad:
Precio:
al proveedor
ER Alpha / Estrogen Receptor Antibody (aa501-550) LS-C117641
LifeSpan Biosciences
catálogo: LS-C117641

total number of products: 181

conejo policlonal
reactividad: humanos
aplicación: western blot, inmunohistoquímica, inmunocitoquímica, immunohistochemistry - paraffin section

Immunofluorescence analysis of A549 cells, using Estrogen Receptor-alpha Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Estrogen Receptor-alpha Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from 293 cells, using Estrogen Receptor-alpha Antibody. The lane on the right is blocked with the synthesized peptide.
cantidad:
Precio:
al proveedor
ER Alpha / Estrogen Receptor Antibody (aa246-273) LS-C167830
LifeSpan Biosciences
catálogo: LS-C167830

total number of products: 181

conejo policlonal
reactividad: humanos
aplicación: western blot, inmunohistoquímica, inmunocitoquímica, immunohistochemistry - paraffin section

ESR1/ER Antibody western blot of MCF-7 cell line lysates (35 ug/lane). The ESR1/ER antibody detected the ESR1/ER protein (arrow).

ESR1/ER Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human breast carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.

Confocal immunofluorescence of ESR1/ER Antibody with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
cantidad:
Precio:
al proveedor
ratón anticuerpo monoclonal MITF
ProMab
catálogo: 31088

total number of products: 9

ratón monoclonal (8F1G5)
reactividad: humanos
aplicación: western blot, ELISA, inmunohistoquímica, inmunocitoquímica, citometría de flujo

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Figure 2:Western blot analysis using MITF mAb against human MITF (AA: 1-114) recombinant protein. (Expected MW is 38.9 kDa)

Figure 3:Western blot analysis using MITF mAb against HEK293 (1) and MITF (AA: 1-114)-hIgGFc transfected HEK293 (2) cell lysate.
cantidad: 0.1 mg
Precio: 341.00 USD
al proveedor
ratón anticuerpo monoclonal ESRRA
ProMab
catálogo: 31091

total number of products: 9

ratón monoclonal (6F1E6)
reactividad: humanos, rata
aplicación: western blot, ELISA, inmunohistoquímica, inmunocitoquímica, citometría de flujo

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Figure 2:Western blot analysis using ESRRA mAb against human ESRRA (AA: 198-376) recombinant protein. (Expected MW is 45.3 kDa)

Figure 3:Western blot analysis using ESRRA mAb against HEK293 (1) and ESRRA (AA: 198-376)-hIgGFc transfected HEK293 (2) cell lysate.
cantidad: 0.1 mg
Precio: 341.00 USD
al proveedor
Mouse Monoclonal Antibody to phospho-Eralpha(Tyr-537)
ProMab
catálogo: 30930

total number of products: 9

ratón monoclonal (3C6C6)
reactividad: humanos
aplicación: western blot, ELISA, inmunohistoquímica, inmunocitoquímica, citometría de flujo

Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Figure 2:Flow cytometric analysis of HeLa cells using Eralpha(Tyr-537) mouse mAb (green) and negative control (red).
cantidad: 0.1 mg
Precio: 341.00 USD
al proveedor
alpha Estrogen Receptor antibody
Biorbyt
catálogo: orb10612

total number of products: 23

conejo policlonal
reactividad: humanos, ratón, rata
aplicación: western blot, ELISA

Immunohistochemical analysis of paraffin embedded submaxillary gland of rats using alpha Estrogen Receptor antibody

IHC-P of rat submandibular gland (alpha Estrogen Receptor antibody at 1:200)

Immunohistrochemical analysis of formalin fixed and paraffin embedded rat submaxillary gland using alpha Estrogen Receptor antibody
cantidad: 100 ug, 200 ug
Precio: 215.0, 350.0 USD
al proveedor
alpha Estrogen Receptor antibody
Biorbyt
catálogo: orb10614

total number of products: 23

conejo policlonal
reactividad: humanos, ratón
aplicación: western blot, ELISA

Immunohistochemical analysis of paraffin-embedded human laryngeal cancer tissue using alpha Estrogen Receptor antibody

IHC-P of human laryngeal cancer tissue (alpha Estrogen Receptor antibody at 1:200)

Immunohistochemical analysis of formalin fixed and paraffin embedded human laryngeal carcinoma using alpha Estrogen Receptor antibody
cantidad: 100 ug, 200 ug
Precio: 215.0, 350.0 USD
al proveedor
alpha Estrogen Receptor antibody
Biorbyt
catálogo: orb14479

total number of products: 23

conejo policlonal
reactividad: humanos, ratón
aplicación: western blot

Western blot analysis of Estrogen Receptor-a(Ab-118) antibody in MCF cells lysates

Confocal immunofluorescent analysis of MCF cells using Estrogen Receptor-a(Ab-118) antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-a(Ab-118) antibody
cantidad: 100 ul
Precio: 191 USD
al proveedor
ER alpha/ESR1 Antibody, Rabbit MAb
Sino Biological
catálogo: 100386-R107

conejo monoclonal (107)
reactividad: humanos
aplicación: western blot

cantidad: 100µL
Precio: 199 USD
al proveedor
ER[1D5]
Medaysis
catálogo: MC0335

ratón monoclonal (1D5)
reactividad: humanos
aplicación: inmunohistoquímica

cantidad: 1 ml
Precio: 540 USD
al proveedor
ER[EP1]
Medaysis
catálogo: RM0092

conejo monoclonal (EP1)
reactividad: humanos
aplicación: inmunohistoquímica

cantidad: 1 ml
Precio: 540 USD
al proveedor
ER[SP1 ]
Medaysis
catálogo: RM0248

conejo monoclonal (Sp1)
reactividad: humanos
aplicación: western blot, inmunohistoquímica, inmunocitoquímica

cantidad: 1 ml
Precio: 600 USD
al proveedor
ER Estrogen receptor
Agrisera
catálogo: AS05 065R

conejo policlonal
reactividad: humanos
aplicación: western blot

cantidad: 100 µg
Precio:
al proveedor
Anti-Estrogen Receptor/ESR1 Picoband Antibody
Boster
catálogo: PB9192

conejo policlonal
reactividad: humanos
aplicación: western blot, inmunohistoquímica

Anti- Estrogen Receptor antibody, PB9192, Western blotting. All lanes: Anti Estrogen Receptor (PB9192) at 0.5ug/ml. WB: Recombinant Human ER Protein 0.5ng. Predicted bind size: 37KD. Observed bind size: 37KD

Anti- Estrogen Receptor antibody, PB9192, Western blotting. All lanes: Anti Estrogen Receptor (PB9192) at 0.5ug/ml. WB: MCF-7 Whole Cell Lysate at 40ug. Predicted bind size: 66KD. Observed bind size: 66KD

Anti- Estrogen Receptor antibody, PB9192, IHC(P). IHC(P): Human Mammary Cancer Tissue
cantidad: 100 ug/vial
Precio: 280 USD
al proveedor
Anti-ER alpha Rabbit Monoclonal Antibody
Boster
catálogo: M00057-2

conejo monoclonal (IE-5)
reactividad: humanos, ratón, rata, perro
aplicación: western blot, inmunohistoquímica, inmunocitoquímica, citometría de flujo, inmunoprecipitación de la cromatina

Western blot analysis of ER alpha expression in (1) MCF7 cell lysate; (2)T47-D cell lysate (M00057-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ESR1 monoclonal antibody (Catalog # M00057-2) overnight at 4Â°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ESR1

Immunohistochemical analysis of paraffin-embedded human cervix carcinoma, using ER alpha Antibody(M00057-2). ESR1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ESR1 Antibody (M00057-2)overnight at 4Â°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37Â°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

IF analysis of immunocytochemical section of MCF7 cells using anti- ER alpha antibody (M00057-2) . ER alpha was detected in immunocytochemical section. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/mL rabbit anti- ER alpha Antibody (M00057-2) overnight at 4 C. DyLightÂ®488 Conjugated Goat AntiRabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used
cantidad: 100 ug/vial
Precio: 299 USD
al proveedor
Anti-Phospho-ER alpha (S118) Rabbit Monoclonal Antibody
Boster
catálogo: P00057

conejo monoclonal (EIG-5)
reactividad: humanos
aplicación: western blot, inmunohistoquímica, inmunocitoquímica

Western blot analysis of Phospho-ER alpha (S118) expression in (1) MCF7 cell lysate; (2) MCF7 cell lysate treated with b-Estradiol and EGF (P00057). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ESR1 monoclonal antibody (Catalog # P00057) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ESR1

IHC analysis of PHOSPHO-ER ALPHA (S118) using anti-PHOSPHO-ER ALPHA (S118) antibody (P00057) on paraffin-embedded human kidney. PHOSPHO-ER ALPHA (S118) was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PHOSPHO-ER ALPHA (S118) Antibody (P00057) overnight at 4Â°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at Western blot analysis of GFP fusion protein expression in (1) 293T cell lysate; (2) 293T cell transfected GFP fusion protein with GFP Antibody(HRP conjugated) (H30939). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GFP monoclonal antibody (Catalog # H30939) overnight at 4â„ƒ, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GFP. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

IF analysis of immunocytochemical section of MCF7 cells using anti- -Phospho-ER alpha (S118) antibody (P00057) . -Phospho-ER alpha (S118) was detected in immunocytochemical section. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/mL rabbit anti- -Phospho-ER alpha (S118) Antibody (P00057) overnight at 4Â°C. DyLightÂ®488 Conjugated Goat AntiRabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37Â°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
cantidad: 100 ug/vial
Precio: 299 USD
al proveedor
ESR1 anticuerpos
StressMarq Biosciences
catálogo: SPC-708D

conejo policlonal
reactividad: humanos
aplicación: western blot, inmunohistoquímica, inmunocitoquímica

cantidad: 100 µg
Precio: 295.00 USD
al proveedor
Estrogen Receptor alpha Antibody (pSer104)
StressMarq Biosciences
catálogo: SPC-1374D

conejo policlonal
reactividad: humanos, ratón, rata
modificaciones : fosforilada
aplicación: western blot, ELISA, inmunohistoquímica

cantidad: 100 µl
Precio: 295.00 USD
al proveedor
Estrogen Receptor alpha Antibody (pSer106)
StressMarq Biosciences
catálogo: SPC-1375D

conejo policlonal
reactividad: humanos, ratón, rata
modificaciones : fosforilada
aplicación: western blot, ELISA, inmunohistoquímica, inmunocitoquímica

cantidad: 100 µl
Precio: 295.00 USD
al proveedor
ESR1 anticuerpos
Rockland Immunochemicals
catálogo: 600-401-BB9

conejo policlonal
reactividad: humanos, ratón, rata
aplicación: western blot, ELISA, inmunohistoquímica

cantidad: 100 µg
Precio: 359.00 USD
al proveedor
Recombinant Anti-Human ESR1 VHH Single Domain Antibody
Creative Biomart
catálogo: NAB-898-VHH

llama recombinant
reactividad: humanos
aplicación: inmunocitoquímica, citometría de flujo

cantidad: 1 mg
Precio:
al proveedor
Estrogen Receptor- alpha Monoclonal Antibody
MyBioSource
catálogo: MBS190147

total number of products: 8

ratón monoclonal
reactividad: humanos
aplicación: western blot, inmunoprecipitación

cantidad: 0.1 mL
Precio: 410 USD
al proveedor
Mouse Monoclonal (IgG1) to Human ER Alpha / Estrogen Receptor
MyBioSource
catálogo: MBS245027

total number of products: 8

ratón monoclonal
reactividad: humanos
aplicación: western blot, inmunohistoquímica

cantidad: 0.05 ml
Precio: 495 USD
al proveedor
Estrogen Receptor RMab
MyBioSource
catálogo: MBS370067

total number of products: 8

conejo monoclonal (RBT11)
reactividad: humanos

cantidad: 5 + Control Slides
Precio: 115 USD
al proveedor
ER conejo policlonal
Wuhan Fine Biotech Co.,Ltd.
catálogo: FNab02822

conejo policlonal
reactividad: humanos
aplicación: western blot, ELISA, inmunohistoquímica

cantidad: 100 ug
Precio: 245USD
al proveedor
ESR1 anticuerpos
Abbexa
catálogo: abx010735

ratón monoclonal
reactividad: humanos
aplicación: western blot, ELISA

cantidad: 100 µl
Precio: 377 USD
al proveedor
Estrogen Receptor alpha (Phospho-Tyr537) Antibody
Abbexa
catálogo: abx012436

conejo policlonal
reactividad: humanos, ratón
aplicación: western blot, ELISA, inmunohistoquímica

cantidad: 100 µg
Precio: 275.5 USD
al proveedor
Estrogen Receptor Alpha (ERa) Antibody
Abbexa
catálogo: abx128152

conejo policlonal
reactividad: humanos
aplicación: western blot, inmunohistoquímica, inmunocitoquímica, inmunoprecipitación

cantidad: 100 µg
Precio: 377 USD
al proveedor
Polyclonal Antibody to Estrogen Receptor alpha
Abeomics
catálogo: 11-7577

conejo policlonal
reactividad: humanos
aplicación: western blot

cantidad: 100 µg
Precio: 240 USD
al proveedor
Monoclonal Antibody to Estrogen Receptor (Clone: C-70)
Abeomics
catálogo: 36-3028

ratón monoclonal (C-70)
reactividad: humanos
aplicación: western blot, inmunohistoquímica

cantidad: 100 µg
Precio: 295 USD
al proveedor
Monoclonal Antibody to Estrogen Receptor (Clone: C-71)
Abeomics
catálogo: 36-3029

ratón monoclonal (C-71)
reactividad: humanos
aplicación: inmunohistoquímica, FACS

cantidad: 100 µg
Precio: 260 USD
al proveedor
Rabbit Polyclonal ER alpha/NR3A1 Antibody
Novus Biologicals
catálogo: NB200-347

total number of products: 11

conejo policlonal
reactividad: humanos
aplicación: western blot, inmunoprecipitación

Proximity Ligation Assay: ER alpha/NR3A1 Antibody [NB200-347] - Secondary-conjugate Duolink in Situ PLA in Hela cells. Goat anti-human DBC1 (NBP2-22294) and rabbit anti-human Estrogen Receptor Alpha (NB200-347) Image merged from DAPI (2ms), FITC/Green (25ms) and Red (200ms) exposures, 40X magnification. Control images may not have counterstained cytoplasm.

Proximity Ligation Assay: ER alpha/NR3A1 Antibody [NB200-347] - Secondary-conjugate Duolink in Situ PLA MCF-7 cells. Goat anti-human POLR2A (NBP2-14859) and rabbit anti-human Estrogen Receptor Alpha (NB200-347). Image merged from DAPI (2ms), FITC/Green (25ms) and TRITC/Orange (250ms) exposures, 40X magnification.

Western Blot: Estrogen Receptor alpha Antibody [NB200-347] - Samples: MCF7 WCE (5, 15, 50 ug for WB; 1 mg for IP, 1/4 of IP loaded). Antibodies: NB 200-344, NB 200-345, NB 200-346, and NB 200-347, each used at 3 ug/ml lysate for IP. ER alpha was blotted (WB and IP) using NB 200-347 (0.1 ug/ml). ECL: 3 minutes.
cantidad: 100 ul
Precio: 379 USD
al proveedor
ER alpha/NR3A1 Antibody (CL1196)
Novus Biologicals
catálogo: NBP2-34478

total number of products: 11

ratón monoclonal (CL1196)
reactividad: humanos
aplicación: western blot, inmunohistoquímica, immunohistochemistry - paraffin section

Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Immunohistochemical staining of human endometrium shows strong nuclear immunoreactivity in glandular cells.

Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Immunohistochemical staining of human fallopian tube shows strong immunoreactivity in epithelial cells.

Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Immunohistochemical staining of human cervix shows strong nuclear positivity in epithelial cells.
cantidad: 0.1 ml
Precio: 429 USD
al proveedor
Mouse Monoclonal ER alpha/NR3A1 Antibody
Novus Biologicals
catálogo: NB300-560

total number of products: 11

ratón monoclonal (33)
reactividad: humanos, ratón, rata, vacas
aplicación: western blot, inmunohistoquímica, inmunocitoquímica, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Western Blot: ER alpha/NR3A1 Antibody (33) [NB300-560] - Analysis of recombinant ER alpha.
cantidad: 100 ug
Precio: 329 USD
al proveedor
Anti-ESR1 (ab2) antibody produced in rabbit
MilliporeSigma
catálogo: SAB2100712

conejo policlonal
reactividad: humanos, perro, vacas
aplicación: western blot, immunohistochemistry - paraffin section
citations: 1

cantidad:
Precio:
al proveedor
anti-Estrogen Receptor (ER) (SP1) Rabbit Monoclonal Primary Antibody
MilliporeSigma
catálogo: 790-4324

conejo monoclonal (Sp1)
reactividad: humanos
aplicación: immunohistochemistry - paraffin section
citations: 1

cantidad:
Precio:
al proveedor
Monoclonal Anti-ESR1 antibody produced in mouse
MilliporeSigma
catálogo: SAB1402766

ratón monoclonal (2F8)
reactividad: humanos
aplicación: western blot

cantidad:
Precio:
al proveedor
ER-alpha
US Biological
catálogo: 030186

total number of products: 31

ratón monoclonal (13B264)
reactividad: humanos

cantidad: 100 ul
Precio: 380 USD
al proveedor
ESR1 (Estrogen Receptor 1)
US Biological
catálogo: 030195

total number of products: 31

ratón monoclonal (13B273)
reactividad: humanos

cantidad: 100 ul
Precio: 436 USD
al proveedor
ESR1 (Estrogen receptor 1)
US Biological
catálogo: 030196

total number of products: 31

ratón monoclonal (13B274)
reactividad: humanos

cantidad: 100 ul
Precio: 473 USD
al proveedor
Estrogen Receptor α (D8H8) Rabbit mAb
Cell Signaling Technology
catálogo: 8644

total number of products: 4

conejo monoclonal (D8H8)
reactividad: humanos, rata
aplicación: western blot, inmunoprecipitación, inmunoprecipitación de la cromatina
citations: 11

cantidad:
Precio:
al proveedor
Estrogen Receptor Antibody, clone B10
EMD Millipore
catálogo: MAB463

total number of products: 7

ratón monoclonal (B10)
reactividad: humanos, rata
aplicación: western blot, ELISA, inmunohistoquímica, immunohistochemistry - paraffin section
citations: 6

cantidad: 100 µL
Precio:
al proveedor
ERalpha
Leica Biosystems
catálogo: NCL-L-ER-6F11

total number of products: 2

ratón monoclonal
reactividad: humanos, rata
aplicación: western blot, inmunohistoquímica, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 15

cantidad:
Precio:
al proveedor
estrogen receptor alpha, ligand binding domain (aa 304-554)
Developmental Studies Hybridoma Bank
catálogo: ERalpha BZ1

total number of products: 2

ratón monoclonal (ERalpha BZ1)
reactividad: humanos
citations: 1

cantidad:
Precio:
al proveedor